The hydrogen exchange core and protein folding
نویسندگان
چکیده
منابع مشابه
Hydrogen exchange methods to study protein folding.
The measurement of amino acid-resolved hydrogen exchange (HX) has provided the most detailed information so far available on the structure and properties of protein folding intermediates. Direct HX measurements can define the structure of tenuous molten globule forms that are generally inaccessible to the usual crystallographic and NMR methods (C. Redfield review in this issue). HX pulse labeli...
متن کاملExperimental approaches to protein folding based on the concept of a slow hydrogen exchange core.
In a review of protein hydrogen exchange, we concluded that the slow exchange core is the folding core. By this we mean that the elements of secondary structure carrying the slowest exchanging backbone amides will tend to be the elements of secondary structure to fold first, that partially folded proteins will tend to be most organized in the core, and that peptides made to mimic the slow excha...
متن کاملProtein folding intermediates and pathways studied by hydrogen exchange.
In order to solve the immensely difficult protein-folding problem, it will be necessary to characterize the barriers that slow folding and the intermediate structures that promote it. Although protein-folding intermediates are not accessible to the usual structural studies, hydrogen exchange (HX) methods have been able to detect and characterize intermediates in both kinetic and equilibrium mod...
متن کاملRelationship between the native-state hydrogen exchange and folding pathways of a four-helix bundle protein.
The hydrogen exchange behavior of a four-helix bundle protein in low concentrations of denaturant reveals some partially unfolded forms that are significantly more stable than the fully unfolded state. Kinetic folding of the protein, however, is apparently two-state in the absence of the accumulation of early folding intermediates. The partially unfolded forms are either as folded as or more fo...
متن کاملStart2Fold: a database of hydrogen/deuterium exchange data on protein folding and stability
Proteins fulfil a wide range of tasks in cells; understanding how they fold into complex three-dimensional (3D) structures and how these structures remain stable while retaining sufficient dynamics for functionality is essential for the interpretation of overall protein behaviour. Since the 1950's, solvent exchange-based methods have been the most powerful experimental means to obtain informati...
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ژورنال
عنوان ژورنال: Protein Science
سال: 1999
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.8.8.1571